Use of Elevated Reverse Transcription Reaction Temperatures in RT-PCR

Use of elevated reverse transcription reaction temperatures in RT-PCR.

The use of reverse transcriptase coupled with the polymerase chain reaction (RT-PCR) has been proven to be a valuable tool in the synthesis and isolation of cDNA clones as well as the quantification of rare messenger RNA species. While a number of different approaches can be used to permit gene-specific cDNA synthesis and amplification, a recurring problem is one of high background signal (i.e....

One-Step Reverse Transcription PCR (RT-PCR). Fatemeh Mehrpooyan*

Reverse transcriptase-polymerase chain reaction (rt-PCR).

Reverse transcriptase polymerase chain reaction (RT-PCR) has become a well-established and powerful molecular technique for studying ribonucleic acids. It is used in medical diagnostics for detecting viral RNA, in hematology and oncology for detecting chimeric transcripts of rearranged genes (1), and in the broad area of research applications in gene expression studies. Since its introduction (...

Messenger RNA Profiling: Body Fluid ID using Multiplex Reverse Transcription-polymerase chain reaction (RT-PCR)

Messenger RNA Profiling: Body Fluid ID using Multiplex Reverse Transcription-polymerase chain reaction (RT-PCR) [DIV]